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Proceedings Paper

Effects of specific inhibitors and low irradiance visible light on the redox cycling of cytochrome c in isolated mitochondria using resonance Raman spectroscopy
Author(s): Josh W. Lalonde; Gary D. Noojin; Nathaniel J. Pope; Samantha M. Powell; Vladislav V. Yakovlev; Michael L. Denton
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Paper Abstract

Photobiomodulation (PBM), once known as low level laser therapy (LLLT), is a technique that uses light in the red and near infrared (NIR) range (600-900 nm) to elicit a beneficial physiological change in tissue. These physiological changes are thought to originate in the mitochondria by altering the metabolic rate for the electron transport chain (ETC). Resonance Raman spectroscopy at 532 nm was used to determine the reduction/oxidation (redox) state of cytochrome c in isolated mitochondria after exposure to low irradiance visible light. Mitochondria from porcine heart tissue were isolated and placed in a succinate buffer and then exposed to NIR (808 nm) light. Some enzymes involved in the ETC were selectively individually blocked and the resonance Raman spectra of cytochrome c redox state was measured before and after being illuminated by light representing photobiomodulation. By combining data on the cytochrome c redox state with the effects of inhibiting important enzymes, we can see what the impact of each enzyme in the ETC has on the effects we see from photobiomodulation. This method provides a better understanding on the underlying effects of photobiomodulation on the mitochondria, which can help direct efforts for targeted effects.

Paper Details

Date Published: 11 March 2020
PDF: 7 pages
Proc. SPIE 11221, Mechanisms of Photobiomodulation Therapy XV, 112210F (11 March 2020); doi: 10.1117/12.2545236
Show Author Affiliations
Josh W. Lalonde, Texas A&M Univ. (United States)
Gary D. Noojin, SAIC (United States)
Nathaniel J. Pope, Oak Ridge Institute of Science and Engineering (United States)
Samantha M. Powell, National Research Council (United States)
Vladislav V. Yakovlev, Texas A&M Univ. (United States)
Michael L. Denton, National Research Council (United States)

Published in SPIE Proceedings Vol. 11221:
Mechanisms of Photobiomodulation Therapy XV
Michael R. Hamblin; James D. Carroll; Praveen Arany, Editor(s)

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