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Proceedings Paper

High-throughput and label-free imaging of individual biological nanoparticles at high-resolution (Conference Presentation)

Paper Abstract

Morphological parameters of biological nanoparticles (BNPs) have strong implications on their fate and functionality in vivo e.g., circulation, biodistribution, and clearance. Although interferometric scattering microscopy modalities have demonstrated the label-free detection of sub-100 nm BNPs including viruses and exosomes; they have an insufficient spatial resolution roughly limited to the illumination wavelength. Here, we introduce computational imaging to interferometric scattering microscopy with asymmetric illumination and demonstrate a two-fold resolution enhancement. We demonstrate high-resolution imaging of nanoparticles across a large field-of-view of 100 µm × 100 µm. This novel imaging platform enables ultrasensitive and label-free morphological visualization of low-index sub-diffraction-limited BNPs in a high-throughput manner at subwavelength resolution.

Paper Details

Date Published: 10 March 2020
Proc. SPIE 11258, Frontiers in Biological Detection: From Nanosensors to Systems XII, 112580H (10 March 2020); doi: 10.1117/12.2545232
Show Author Affiliations
Celalettin Yurdakul, Boston Univ. (United States)
Oguzhan Avci, Boston Univ. (United States)
Alex C. Matlock, Boston Univ. (United States)
Lei Tian, Boston Univ. (United States)
Selim Ünlü, Boston Univ. (United States)

Published in SPIE Proceedings Vol. 11258:
Frontiers in Biological Detection: From Nanosensors to Systems XII
Amos Danielli; Benjamin L. Miller; Sharon M. Weiss, Editor(s)

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