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Proceedings Paper

Flexible and uniform illumination for wide field microscopy from TIRF to epiflourescence excitation (Conference Presentation)
Author(s): Adrien Mau; Nicolas Bourg; Sandrine Leveque-Fort

Paper Abstract

We propose a new hybrid scanning/wide field excitation implementation, developed on a classical inverted wide field fluorescence microscope. Compatible from epifluorescence to TIRF excitation it provides flexible and uniform illumination over wide field of views (200x200 µm²), with uniform sectioning depth. This permits to enhance TIRF imaging and control photobleaching in classical microscopy. In single molecule localization microscopy, one can enhance the localization precision by balancing the density of activated fluorophores and the photon count, and extract information from many quantitative datas thanks to the wide uniform illumination. The required laser power to achieve efficient blinking is also considerably reduced.

Paper Details

Date Published: 9 March 2020
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Proc. SPIE 11245, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII, 112450B (9 March 2020); doi: 10.1117/12.2545231
Show Author Affiliations
Adrien Mau, Institut des Sciences Moléculaires d'Orsay (France)
Nicolas Bourg, Abbelight (France)
Sandrine Leveque-Fort, Institut des Sciences Moléculaires d'Orsay (France)


Published in SPIE Proceedings Vol. 11245:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII
Thomas G. Brown; Tony Wilson; Laura Waller, Editor(s)

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