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Proceedings Paper

Probing the mechanisms of infrared neural stimulation with stimulated Raman scattering microscopy (Conference Presentation)
Author(s): Wilson R. Adams; Rekha Gautam; Graham A. Throckmorton; Laura E. Masson; Jeremy B. Ford; John Logan Jenkins; E. Duco Jansen; Anita Mahadevan-Jansen

Paper Abstract

Stimulated Raman scattering (SRS) offers a drastic speed advantage over conventional vibrational spectroscopic imaging techniques – making it ideal for studying fast biochemical dynamics. We developed an experimental paradigm that applies spectral stimulated Raman scattering (SRS) imaging to study the mechanisms of infrared (IR) photostimulation of neuronal cells. Infrared neural stimulation (INS) is a label-free optical neuromodulation technique with high spatial and temporal precision. Using SRS, changes in lipid and water vibrational signatures in live cells during INS were observed, suggesting that lipid membrane deformation accompanies IR exposure. The speeds afforded by SRS enables unprecedented observation of fast cellular biophysical dynamics.

Paper Details

Date Published: 10 March 2020
Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 1125204 (10 March 2020); doi: 10.1117/12.2545228
Show Author Affiliations
Wilson R. Adams, Vanderbilt Univ. (United States)
Rekha Gautam, Vanderbilt Univ. (United States)
Graham A. Throckmorton, Vanderbilt Univ. (United States)
Laura E. Masson, Vanderbilt Univ. (United States)
Jeremy B. Ford, Vanderbilt Univ. (United States)
John Logan Jenkins, Vanderbilt Univ. (United States)
E. Duco Jansen, Vanderbilt Univ. (United States)
Anita Mahadevan-Jansen, Vanderbilt Univ. (United States)

Published in SPIE Proceedings Vol. 11252:
Advanced Chemical Microscopy for Life Science and Translational Medicine
Ji-Xin Cheng; Wei Min; Garth J. Simpson, Editor(s)

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