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Proceedings Paper

Hyper-Raman spectroscopy of biologically important molecules
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Paper Abstract

Raman spectroscopy is an essential optical tool for molecular fingerprints. The vibrational modes of biologically important molecules including proteins, nucleic acids and lipids have been studied to provide insight into their structure as well as insight into the metabolic processes and biomarker expression of cells. To explore hyper-Raman scattering as a complementary technique to Raman scattering, we build a laser system that can perform Raman and hyper-Raman scattering studies using a single setup. Using three amplification stages we are able to generate 8 ps, 1064 nm pulses at repetition rates up to 30 MHz. Converting the 1064 nm source laser to 532 nm, we achieve fast hyper-Raman detection and collect our spectrum with a commercial spectrometer and CCD. Using a single optical setup, we collect and compare Raman spectra at 532 nm to hyper-Raman spectra at 266 nm for water, ethanol and L-tartaric acid. Furthermore, we observe changes in the hyper-Raman peak intensities of an aqueous L-tartaric acid solution when selecting different laser repetition rates highlighting the need to control laser power and repetition rate to identify and mitigate thermal effects in biomolecules.

Paper Details

Date Published: 21 February 2020
PDF: 8 pages
Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 112520V (21 February 2020); doi: 10.1117/12.2545215
Show Author Affiliations
Christopher B. Marble, Texas A&M Univ. (United States)
Xingqi Xu, Texas A&M Univ. (United States)
Zhejiang Univ. (China)
Kassie S. Marble, Texas A&M Univ. (United States)
Georgi I. Petrov, Texas A&M Univ. (United States)
Dawei Wang, Zhejiang Univ. (China)
Vladislav V. Yakovlev, Texas A&M Univ. (United States)

Published in SPIE Proceedings Vol. 11252:
Advanced Chemical Microscopy for Life Science and Translational Medicine
Ji-Xin Cheng; Wei Min; Garth J. Simpson, Editor(s)

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