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Proceedings Paper

Combining 3D single molecule localization strategies and sprectral demultiplexing for bioimaging (Conference Presentation)

Paper Abstract

DAISY combined Supercritical Angle Fluorescence (SAF) detection in order to provide an absolute axial reference to a point spread function engineering approach . The dual view optical setup permits to introduce a strong astigmatism and decouple lateral and axial information, thus extending the axial performances without degrading the lateral precision. This technique provides 3D absolute information over a 1-µm capture range above the glass coverslip and an axial localization precision down to 15 nm with minimal loss of lateral resolution and little sensitivity to field aberrations. We will present sequential and simultaneous up to 3 color 3D imaging in cells.

Paper Details

Date Published: 9 March 2020
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Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 112460I (9 March 2020); doi: 10.1117/12.2545210
Show Author Affiliations
Clément Cabriel, Institut des Sciences Moléculaires d'Orsay (France)
Nicolas Bourg, Institut des Sciences Moléculaires d'Orsay (France)
Pierre Jouchet, Institut des Sciences Moléculaires d'Orsay (France)
Guillaume Dupuis, Institut des Sciences Moléculaires d'Orsay (France)
Cyndelia Guillaume, Abbelight (France)
Aurélie Baron, Institut de Chimie des Substances Naturelles (France)
Marie-Ange Badet-Denisot, Ctr. de Recherche de Gif-sur-Yvette (France)
Boris Vauzeilles, Institut de chimie moléculaire et des matériaux d'Orsay (France)
Christophe Leterrier, Aix-Marseille Univ. (Finland)
Emmanuel Fort, Institut Langevin Ondes et Images (France)
Sandrine Leveque-Fort, Institut des Sciences Moléculaires d'Orsay (France)


Published in SPIE Proceedings Vol. 11246:
Single Molecule Spectroscopy and Superresolution Imaging XIII
Ingo Gregor; Felix Koberling; Rainer Erdmann, Editor(s)

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