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Proceedings Paper

3D single molecule localization microscopy based on time modulated illumination (Conference Presentation)
Author(s): Pierre Jouchet; Clément Cabriel; Nicolas Bourg; Marion Bardou; Christian Poüs; Emmanuel Fort; Sandrine Leveque-Fort

Paper Abstract

We propose Modloc, a new single molecule localization strategy based on a time signature. By shifting a structured excitation pattern, it induces a time modulated emission of the fluorophores where the phase holds its position. An efficient demodulation optical assembly compatible with short emitters On-time has been designed. Performances of ModLoc, will be discussed, in particular its 2 fold lateral precision improvement and its robustness to optical aberrations. By applying the modulation in a tilted direction, we will show that an almost isotropic 3D localization (~11 nm) is obtained over the whole capture range up to 30 µm in depth

Paper Details

Date Published: 9 March 2020
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Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 112460Q (9 March 2020); doi: 10.1117/12.2545196
Show Author Affiliations
Pierre Jouchet, Institut des Sciences Moléculaires d'Orsay (France)
Clément Cabriel, Institut des Sciences Moléculaires d'Orsay (France)
Nicolas Bourg, Institut des Sciences Moléculaires d'Orsay (France)
Marion Bardou, Institut des Sciences Moléculaires d'Orsay (France)
Christian Poüs, Institut Paris Saclay d’Innovation Thérapeutique (France)
Emmanuel Fort, Institut Langevin Ondes et Images (France)
Sandrine Leveque-Fort, Institut des Sciences Moléculaires d'Orsay (France)


Published in SPIE Proceedings Vol. 11246:
Single Molecule Spectroscopy and Superresolution Imaging XIII
Ingo Gregor; Felix Koberling; Rainer Erdmann, Editor(s)

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