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Proceedings Paper

A paired-agent fluorescent molecular imaging strategy for quantifying antibody drug target engagement in in vivo window chamber xenograft models
Author(s): Elif Kayaalp-Nalbant; Cody Rounds; Negar Sadeghipour; Boyu Meng; Margaret R. Folaron; Chandrika Haldar; Rendall R. Strawbridge; Kimberley S. Samkoe; Scott C. Davis; Kenneth M. Tichauer
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Paper Abstract

A paired-agent fluorescent molecular imaging strategy is presented as a method to measure drug target engagement in whole tumor imaging. The protocol involves dynamic imaging of a pair of targeted and control imaging agents prior to and following antibody therapy. Simulations demonstrated that antibody “drug target engagement” can be estimated within a 15%-error over a wide range of tumor physiology (blood flow, vascular permeability, target density) and antibody characteristics (affinity, binding rates). Experimental results demonstrated the first in vivo detection of binding site barrier, highlighting the potential for this methodology to provide novel insights in drug distribution/binding imaging.

Paper Details

Date Published: 19 February 2020
PDF: 4 pages
Proc. SPIE 11219, Visualizing and Quantifying Drug Distribution in Tissue IV, 112190G (19 February 2020); doi: 10.1117/12.2545182
Show Author Affiliations
Elif Kayaalp-Nalbant, Illinois Institute of Technology (United States)
Cody Rounds, Illinois Institute of Technology (United States)
Negar Sadeghipour, Illinois Institute of Technology (United States)
Boyu Meng, Dartmouth College (United States)
Margaret R. Folaron, Dartmouth College (United States)
Chandrika Haldar, Illinois Institute of Technology (United States)
Rendall R. Strawbridge, Dartmouth College (United States)
Kimberley S. Samkoe, Dartmouth College (United States)
Scott C. Davis, Dartmouth College (United States)
Kenneth M. Tichauer, Illinois Institute of Technology (United States)


Published in SPIE Proceedings Vol. 11219:
Visualizing and Quantifying Drug Distribution in Tissue IV
Kin Foong Chan; Conor L. Evans, Editor(s)

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