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Proceedings Paper

Quantitative infrared photothermal microscopy
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Paper Abstract

Infrared photothermal heterodyne imaging (IR-PHI) represents a convenient, table top approach for conducting super-resolution imaging and spectroscopy throughout the all-important mid infrared (MIR) spectral region. Although IR-PHI provides label-free, super-resolution MIR absorption information, it is not quantitative. In this study, we establish quantitative relationships between observed IR-PHI signals and relevant photothermal parameters of investigated specimens. Specifically, we conduct a size series analysis of different radii polystyrene (PS) beads to quantitatively link IR-PHI signal contrast to specimen heat capacity, thermo-optic coefficient, MIR peak absorption cross-section, and scattering cross-section at IR-PHI’s probe wavelength.

Paper Details

Date Published: 13 February 2020
PDF: 7 pages
Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 1124613 (13 February 2020);
Show Author Affiliations
Ilia M. Pavlovetc, Univ. of Notre Dame (United States)
Eduard A. Podshivaylov, M.V. Lomonosov Moscow State Univ. (Russian Federation)
Pavel A. Frantsuzov, Voevodsky Institute of Chemical Kinetics and Combustion (Russian Federation)
Gregory V. Hartland, Univ. of Notre Dame (United States)
Masaru Kuno, Univ. of Notre Dame (United States)


Published in SPIE Proceedings Vol. 11246:
Single Molecule Spectroscopy and Superresolution Imaging XIII
Ingo Gregor; Felix Koberling; Rainer Erdmann, Editor(s)

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