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Proceedings Paper

Highly sensitive and reliable plasmonic nanoparticle-based digital cytometry for quantification of MUC16 binding on the surface of leukocytes (Conference Presentation)
Author(s): Sinyoung Jeong; Germán González; Nicholas Nowell; Lauren A. Austin; Jawad Hoballah; Fatima Mubarak; Arvinder Kapur; Manish S. Patankar; Daniel W. Cramer; Petra Krauledat; W. Peter Hansen; Conor L. Evans

Paper Abstract

We recently developed a digital cytometric assay using plasmonic nanoparticles to quantify the bound ovarian cancer marker (CA125) on single cells. We demonstrate the quantification capability of our novel digital cytometry method by discriminating the different MUC16 binding levels on the PBMCs between healthy donors and EOC patients. Furthermore, we tracked the amount of surface-bound MUC16 on the EOC patient’s PBMCs over 17 months with 1-month time intervals. Initial data from our cytometric technique shows it to be a quantitative tool that can provide new information connecting leukocyte-bound MUC16 to the time course of ovarian cancer.

Paper Details

Date Published: 10 March 2020
Proc. SPIE 11254, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII, 112540R (10 March 2020); doi: 10.1117/12.2545088
Show Author Affiliations
Sinyoung Jeong, Massachusetts General Hospital (United States)
Germán González, PNP Research, LLC (United States)
Nicholas Nowell, PNP Research, LLC (United States)
Lauren A. Austin, Massachusetts General Hospital (United States)
Jawad Hoballah, PNP Research Corp. LLC (United States)
Fatima Mubarak, Massachusetts General Hospital (United States)
Arvinder Kapur, Univ. of Wisconsin-Madison (United States)
Manish S. Patankar, Univ. of Wisconsin-Madison (United States)
Daniel W. Cramer, Brigham and Women's Hospital (United States)
Petra Krauledat, PNP Research Corp. LLC (United States)
W. Peter Hansen, PNP Research Corp. LLC (United States)
Conor L. Evans, Massachusetts General Hospital (United States)

Published in SPIE Proceedings Vol. 11254:
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII
Dror Fixler; Ewa M. Goldys, Editor(s)

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