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Proceedings Paper

Inkjet dispense SERS (ID-SERS) for quantitative analysis and bacterial detection
Author(s): Fausto D'Apuzzo; Raghuvir N. Sengupta; Milo Overbay; Jason Aronoff; Anita Rogacs; Steven J. Barcelo
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Paper Abstract

Surface-Enhanced Raman Scattering (SERS) has emerged as a powerful technique for sensitive detection of a wide variety of substances including drugs, pesticides, and biological molecules. Despite its potential as a broadly applicable detection strategy, quantitative studies using SERS have been hindered by substrate performance variability. We recently reported an in situ calibration method that merges inkjet dispense with SERS (ID-SERS), yielding quantitative measurements with Relative Standard Deviation (RSD) values below 2%. In this work, we provide a conceptual review of ID-SERS, highlighting the advantages of using inkjet to pattern microdroplets (28 pL) on the surface of a single SERS sensor and produce standard curves on a single SERS sensor. We subsequently explore the application potential of IDSERS by producing ID-SERS response curves for adenine and pyocyanin, two well-known metabolites that are biomarkers for bacterial detection. We then report preliminary results that suggest that ID-SERS can be harnessed to detect time-dependent changes in the spectra of metabolites released by bacteria under nutrient-deprived conditions. The ability to use ID-SERS to monitor the time-evolution in the SERS spectra of nutrient-deprived bacteria has implications for developing a SERS-based method for antibiotic susceptibility testing. More generally, we believe that the benefits afforded by ID-SERS will facilitate the development of reproducible plasmonic sensing for real-world applications.

Paper Details

Date Published: 21 February 2020
PDF: 6 pages
Proc. SPIE 11257, Plasmonics in Biology and Medicine XVII, 112570X (21 February 2020); doi: 10.1117/12.2545084
Show Author Affiliations
Fausto D'Apuzzo, HP, Inc. (United States)
Raghuvir N. Sengupta, HP, Inc. (United States)
Milo Overbay, HP, Inc. (United States)
Jason Aronoff, HP, Inc. (United States)
Anita Rogacs, HP, Inc. (United States)
Steven J. Barcelo, HP, Inc. (United States)


Published in SPIE Proceedings Vol. 11257:
Plasmonics in Biology and Medicine XVII
Tuan Vo-Dinh; Ho-Pui A. Ho; Krishanu Ray, Editor(s)

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