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Proceedings Paper

Electronically controllable multiple line-scanning confocal microscopy using digital micromirror device (Conference Presentation)
Author(s): Chun-Hung Weng; Kyu Young Han

Paper Abstract

We propose the multiple focused inclined beams in conjunction a highly efficient detection system and electronically controllable confocal slit array in the fluorescence microscope system to obtain low photodamage, good optical sectioning, video-rate, and high-sensitivity imaging. To do so, we use a digital micro-mirror to generate the multiple beams and to form the detection slit synchronously. This provides the high resolution, the capability to image thick sample and the longer observation time while maintaining a similar imaging speed. By directly programming the digital micro-mirror we could correct the anisotropic beam shape and suppress the background.

Paper Details

Date Published: 9 March 2020
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Proc. SPIE 11245, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII, 1124513 (9 March 2020); doi: 10.1117/12.2545072
Show Author Affiliations
Chun-Hung Weng, CREOL, The College of Optics and Photonics, Univ. of Central Florida (United States)
Kyu Young Han, CREOL, The College of Optics and Photonics, Univ. of Central Florida (United States)


Published in SPIE Proceedings Vol. 11245:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII
Thomas G. Brown; Tony Wilson; Laura Waller, Editor(s)

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