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Proceedings Paper

Characterizing the refractive index auto-correlation function from whole cells using interferometric microscopy (Conference Presentation)
Author(s): Aya Eid; Vadim Backman; Allen Taflove; Adam Eshein; Yue Li; Ranya Virk

Paper Abstract

The hyperspectral, interferometric microscopy technique, PWS has demonstrated the ability to measure variance in the nanoscale refractive index (Σ) of chromatin – the macromolecular assembly containing most of a cell’s genetic material. However, the question arises: how does Σ relate to the physical distribution of mass in chromatin, and specifically the organization of chromatin packing. We developed an analytical framework to relate Σ to the mass-density autocorrelation function – which can fully describe the distribution of mass and is characterized by D. This relationship was validated numerically using the rigorous modelling technique FDTD and experimentally with PWS and Chromatin Electron Microscopy (ChromEM).

Paper Details

Date Published: 10 March 2020
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Proc. SPIE 11253, Biomedical Applications of Light Scattering X, 112530G (10 March 2020); doi: 10.1117/12.2545053
Show Author Affiliations
Aya Eid, Northwestern Univ. (United States)
Vadim Backman, Northwestern Univ. (United States)
Allen Taflove, Northwestern Univ. (United States)
Adam Eshein, Northwestern Univ. (United States)
Yue Li, Northwestern Univ. (United States)
Ranya Virk, Northwestern Univ. (United States)


Published in SPIE Proceedings Vol. 11253:
Biomedical Applications of Light Scattering X
Adam Wax; Vadim Backman, Editor(s)

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