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Proceedings Paper

High-throughput multimodal Raman-fluorescence flow cytometry (Conference Presentation)
Author(s): Matthew Lindley; Kotaro Hiramatsu; Fukashi Shibata; Tsuyoshi Takeshita; Shigeyuki Kawano; Keisuke Goda

Paper Abstract

We present a high-throughput multimodal Raman-fluorescence flow cytometer that produces simultaneous Raman and fluorescence excitation spectra at a demonstrated throughput of 100 events per second. Our method combines Fourier-transform coherent anti-Stokes Raman scattering with Fourier-transform two-photon excitation fluorescence, which produces broadband Raman spectra that span the biological fingerprint region and single-detector multicolor fluorescence via spectral analysis. As a proof-of-principle demonstration, we perform multimodal label-free analysis of stress response in the microalga Haematococcus pluvialis by Raman measurement of its secondary metabolite astaxanthin and two-color fluorescence measurement of chlorophyll and fluorescent chlorophyll catabolites under different stress conditions.

Paper Details

Date Published: 10 March 2020
Proc. SPIE 11250, High-Speed Biomedical Imaging and Spectroscopy V, 112500S (10 March 2020); doi: 10.1117/12.2545022
Show Author Affiliations
Matthew Lindley, The Univ. of Tokyo (Japan)
Kotaro Hiramatsu, Research Ctr. for Spectrochemistry, The Univ. of Tokyo (Japan)
PRESTO, Japan Science and Technology Agency (Japan)
Fukashi Shibata, Algal Bio Co., Ltd. (Japan)
Tsuyoshi Takeshita, Algal Bio Co., Ltd. (Japan)
Shigeyuki Kawano, The Univ. of Tokyo (Japan)
Keisuke Goda, The Univ. of Tokyo (Japan)
Univ. of California, Los Angeles (United States)
Institute of Technological Sciences, Wuhan Univ. (China)

Published in SPIE Proceedings Vol. 11250:
High-Speed Biomedical Imaging and Spectroscopy V
Kevin K. Tsia; Keisuke Goda, Editor(s)

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