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Proceedings Paper

Simple, rapid and cost-effective drug-susceptibility testing of leukemia by intelligent whole-blood imaging flow cytometry (Conference Presentation)
Author(s): Hirofumi Kobayashi; Cheng Lei; Yi Wu; Chun-Jung Huamg; Atsushi Yasumoto; Masahiro Jona; Wenxuan Li; Yaxiaer Yalikun; Baoshan Guo; Chia-Wei Sun; Yo Tanaka; Makoto Yamada; Yutaka Yatomi; Keisuke Goda

Paper Abstract

We present an extreme-throughput (>1 million cells per second) imaging flow cytometer with deep learning to achieve a highly simple, rapid, and cost-effective liquid biopsy for ex-vivo drug-susceptibility testing of leukemia. The drug resistance of leukemia cells was detected in whole blood with only 24-hour drug treatment without hemolysis or dilution, making the sample preparation extremely simple, rapid and cost-effective. Our method also accurately evaluated the drug susceptibility of white blood cells from untreated patients with acute lymphoblastic leukemia, holding great promise for affordable precision medicine.

Paper Details

Date Published: 10 March 2020
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Proc. SPIE 11250, High-Speed Biomedical Imaging and Spectroscopy V, 112500Y (10 March 2020); doi: 10.1117/12.2545017
Show Author Affiliations
Hirofumi Kobayashi, The Univ. of Tokyo (Japan)
Chan Zuckerberg Biohub (United States)
Cheng Lei, The Univ. of Tokyo (Japan)
Wuhan Univ. (China)
Yi Wu, The Univ. of Tokyo (Japan)
Univ. of Toronto (Canada)
Chun-Jung Huamg, The Univ. of Tokyo (Japan)
National Chiao Tung Univ. (Taiwan)
Atsushi Yasumoto, The Univ. of Tokyo (Japan)
Masahiro Jona, The Univ. of Tokyo (Japan)
Wenxuan Li, Carnegie Mellon Univ. (United States)
Yaxiaer Yalikun, Ctr. for Biosystems Dynamics Research, RIKEN (Japan)
Baoshan Guo, The Univ. of Tokyo (Japan)
Chia-Wei Sun, National Chiao Tung Univ. (Taiwan)
Yo Tanaka, Ctr. for Biosystems Dynamics Research, RIKEN (Japan)
Makoto Yamada, Ctr. for Advanced Intelligence Project, RIKEN (Japan)
Yutaka Yatomi, The Univ. of Tokyo (Japan)
Keisuke Goda, The Univ. of Tokyo (Japan)
Wuhan Univ. (China)
Univ. of California, Los Angeles (United States)


Published in SPIE Proceedings Vol. 11250:
High-Speed Biomedical Imaging and Spectroscopy V
Kevin K. Tsia; Keisuke Goda, Editor(s)

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