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Proceedings Paper

A practical theoretical framework for optimizing spontaneous super-resolution on confocal microscopes (Conference Presentation)
Author(s): Martin Ploschner; Denitza Denkova; Minakshi Das; Lindsay M. Parker; Xianlin Zheng; Yiqing Lu; Antony Orth; Nicolle H. Packer; James A. Piper

Paper Abstract

Recently, we realized a simple technique, which spontaneously bypasses the diffraction limit in a conventional confocal microscope by exploiting super-linear effects in nanoparticle bio-markers: super-linear excitation-emission (SEE) microscopy. Here, we present a theoretical framework and its practical implementation for optimizing and expanding this technique. We accurately predict the expected 3D super-resolution by accounting for all crucial parameters affecting the resolution: the empirically measured/modelled excitation-emission curve, the filling factor of the microscope objective back pupil, the polarization and the pinhole setting. The presented theoretical framework is a practical tool, which enables end-users to augment their own confocal microscopes with super-resolution capabilities.

Paper Details

Date Published: 10 March 2020
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Proc. SPIE 11254, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII, 112540E (10 March 2020); doi: 10.1117/12.2544972
Show Author Affiliations
Martin Ploschner, ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia)
Macquarie Univ. (Australia)
The Univ. of Queensland (Australia)
Denitza Denkova, ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia)
Macquarie Univ. (Australia)
Institute for Bioengineering of Catalonia (Spain)
Minakshi Das, ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia)
Macquarie Univ. (Australia)
Lindsay M. Parker, ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia)
Macquarie Univ. (Australia)
Xianlin Zheng, ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia)
Macquarie Univ. (Australia)
Yiqing Lu, ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia)
Macquarie Univ. (Australia)
Antony Orth, ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia)
RMIT Univ. (Australia)
Nicolle H. Packer, ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia)
Macquarie Univ. (Australia)
Institute for Glycomics, Griffith Univ. (Australia)
James A. Piper, ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia)
Macquarie Univ. (Australia)


Published in SPIE Proceedings Vol. 11254:
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII
Dror Fixler; Ewa M. Goldys, Editor(s)

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