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Proceedings Paper

Synchronized subharmonic modulation in stimulated emission microscopy (Conference Presentation)
Author(s): Fu-Jen Kao

Paper Abstract

In this work, we have demonstrated a stimulated emission (SE) based pump-probe microscopy with subharmonic modulation and synchronization on the pump and the probe lasers. Critically, a high frequency divider circuit divides the repetition frequency (76 MHz) of the probe laser (Ti:sapphire) to the half repetition frequency (38 MHz), which in turn drives the pump laser (pulsed gain-switched diode laser) synchronously and provides the reference signal for lock-in detection. In this way, the highest possible modulation frequency can be achieved for lock-in detection with shot noise limited sensitivity. The greatly shortened time constant (< 0.1 ms) further improved imaging speed. Over an order of magnitude improvement in signal-to-noise ratio is achieved, when compared with conventional lock-in detection implement.

Paper Details

Date Published: 9 March 2020
Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112441E (9 March 2020); doi: 10.1117/12.2544936
Show Author Affiliations
Fu-Jen Kao, National Yang-Ming Univ. (Taiwan)

Published in SPIE Proceedings Vol. 11244:
Multiphoton Microscopy in the Biomedical Sciences XX
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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