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Proceedings Paper

Multiscale SHG analysis of collagen architecture alterations in human idiopathic pulmonary fibrosis (Conference Presentation)
Author(s): Paul J. Campagnola; Darian S. James; Zachary Alden; Hsin-Yu Chang; Mark Jones; Nathan Sandbo

Paper Abstract

We use SHG microscopy to study the collagen alterations in idiopathic pulmonary fibrosis (IPF) in ex vivo human tissues and in vitro spheroid models. We found the collagen fibril and macromolecular structures are altered in IPF, consistent with decreased organization. Further, machine learning based texture analysis successfully classified the fiber morphology at near 100% accuracy. To gain insight into the underlying mechanisms of these alterations, we grew IPF based spheroids under different crosslinking conditions (increased or inhibited). SHG polarization measurements (linear and SHG-CD) indicated that increased crosslinking altered the collagen helical structure similar to those of the human tissues.

Paper Details

Date Published: 9 March 2020
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Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 1124412 (9 March 2020); doi: 10.1117/12.2544902
Show Author Affiliations
Paul J. Campagnola, Univ. of Wisconsin-Madison (United States)
Darian S. James, Univ. of Wisconsin-Madison (United States)
Zachary Alden, Univ. of Wisconsin-Madison (United States)
Hsin-Yu Chang, Univ. of Wisconsin-Madison (United States)
Mark Jones, Univ. of Southampton (United Kingdom)
Nathan Sandbo, Univ. of Wisconsin-Madison (United States)


Published in SPIE Proceedings Vol. 11244:
Multiphoton Microscopy in the Biomedical Sciences XX
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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