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Proceedings Paper

High-speed super-multiplex organelle imaging
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Paper Abstract

Among various optical methods, fluorescence imaging has been the most widely exploited thanks to its superior sensitivity and specificity, but the resolvable colors are restricted to 2-5 colors because of the intrinsically broad and featureless spectra. Recently, this fluorescent “color barrier” was broken and super-multiplex optical imaging became possible taking advantage of well-designed Raman probes. However, the acquisition of the super-multiplex images is still relatively slow which impedes wider applications. Here, we demonstrate fast super-multiplex organelle imaging with high-speed color switching and acquisition, which accelerates the imaging speed by 2 orders of magnitude. We applied it in imaging cytometry, tracing mitosis and fast organelle motions in live cells. We anticipate that high-speed supermultiplex optical imaging can expand to a much wider field of biological researches.

Paper Details

Date Published: 21 February 2020
PDF: 5 pages
Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 112521C (21 February 2020); doi: 10.1117/12.2544808
Show Author Affiliations
Jingwen Shou, The Univ. of Tokyo (Japan)
Fanghao Hu, Columbia Univ. (United States)
Robert Oda, The Univ. of Tokyo (Japan)
Univ. of Hawai'i at Manoa (United States)
John A. Burns School of Medicine (United States)
Wei Min, Columbia Univ. (United States)
Yasuyuki Ozeki, The Univ. of Tokyo (Japan)

Published in SPIE Proceedings Vol. 11252:
Advanced Chemical Microscopy for Life Science and Translational Medicine
Ji-Xin Cheng; Wei Min; Garth J. Simpson, Editor(s)

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