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Proceedings Paper

Multiphoton FLIM is gaining ground as a clinical tool (Conference Presentation)

Paper Abstract

Multiphoton fluorescence lifetime imaging (FLIM) is gaining ground as a non-invasive and very sensitive method in life sciences, and even as a clinical tool. First clinical devices employing FLIM are on the market, e.g. MPTflex. A hot topic is using metabolic imaging to investigate melanoma lesions (Fig.1). This method utilizes imaging of the ratio of the amounts of the free and protein-bound forms of the intracellular autofluorescent metabolic co-enzyme nicotinamide adenine dinucleotide (NADH) [1,2,3,4]. In another study, we investigated safety aspects of nanoparticle based sun screens. Multiphoton FLIM enables tracing of nanoparticles after application on the skin [5]. Furthermore, in case of penetration into the viable epidermis metabolic imaging can be employed to investigate toxicity on skin cells [6].

Paper Details

Date Published: 9 March 2020
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Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112441Q (9 March 2020); doi: 10.1117/12.2544795
Show Author Affiliations
Hauke Studier, Becker & Hickl GmbH (Germany)
Yousuf S. Mohammed, The Univ. of Queensland (Australia)
Michael S. Roberts, The Univ. of Queensland (Australia)
Amy Holmes, Univ. of South Australia (Australia)
Michael Pastore, Univ. of South Australia (Australia)
Wolfgang Becker, Becker & Hickl GmbH (Germany)


Published in SPIE Proceedings Vol. 11244:
Multiphoton Microscopy in the Biomedical Sciences XX
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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