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Proceedings Paper

Effect of partial coherent illumination on Fourier ptychography
Author(s): Alexander Heemels; Temitope Agbana; Silvania Pereira; Jan Carel Diehl; Michel Verhaegen; Gleb Vdovin
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Paper Abstract

Fourier Ptychography is a computational imaging technique able to decouple high resolution from wide field of view, bypassing the diffraction limit of the microscope. Since it does not rely on high precision mechanics or fluorescent imaging, it is of practical interest for implementation in low scale devices. Despite its gains, realizing a functional low-cost setup working at the theoretical limits is challenging due to many factors causing discrepancies between theory and practice. Misalignment of the light emitting diode array (LED-array), optical system aberrations and use of partial coherent sources are common issues which have been addressed with calibration algorithms. However, physical interpretation of how these factors influence the algorithm and cause mismatches between theory and practice has had little attention so far. This work provides a discussion based on simulation results on the effect of the partial coherence of the source. From obtained results, an optimal set of LEDs for data acquisition is described which avoids degeneracy caused by partial coherence and is based on the numerical aperture (NA) of the objective and source parameters such as bandwidth and size.

Paper Details

Date Published: 20 February 2020
PDF: 7 pages
Proc. SPIE 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020, 112511K (20 February 2020); doi: 10.1117/12.2544766
Show Author Affiliations
Alexander Heemels, Technische Univ. Delft (Netherlands)
Temitope Agbana, Technische Univ. Delft (Netherlands)
Silvania Pereira, Technische Univ. Delft (Netherlands)
Jan Carel Diehl, Technische Univ. Delft (Netherlands)
Michel Verhaegen, Technische Univ. Delft (Netherlands)
Gleb Vdovin, Technische Univ. Delft (Netherlands)

Published in SPIE Proceedings Vol. 11251:
Label-free Biomedical Imaging and Sensing (LBIS) 2020
Natan T. Shaked; Oliver Hayden, Editor(s)

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