We propose to study the behavior of vesicles at the intracellular scale with a non-invasive quantitative phase imaging technology which enables to follow vesicles during a large amount of time without modifying their behavior. We developed specific tools to localize and track cells that we compare to fluorescence-based tracking method. We studied their interaction with the cytoskeleton by inhibiting it using drugs and we implemented statistical tools to quantify and understand intracellular processes at the vesicular level. The resolution and the high sensitivity brought by the High Definition wave front sensor we developed enables to follow all individual vesicles through time follow up, while imaging the whole cell.

Date Published: 11 March 2020
PDF
Proc. SPIE 11249, Quantitative Phase Imaging VI, 112490W (11 March 2020); doi: 10.1117/12.2544711

Published in SPIE Proceedings Vol. 11249:
Quantitative Phase Imaging VI
Yang Liu; Gabriel Popescu; YongKeun Park, Editor(s)