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Proceedings Paper

Stress and repair in retinal pigmented epithelium cell culture imaged with dynamic full-field OCT (Conference Presentation)

Paper Abstract

Dynamic FFOCT allows us to see the sub-cellular motion of biological samples. We are able to follow the evolution in the same plane of biological samples for hours thanks to an autofocus procedure. RPE cells are involved in the integrity of retina and vision. We performed a linear scratch assay in RPE cell culture with a surgical scalpel blade, inducing border cell migration at 20.8 µm/h to close the scratch. We also recorded motility of microvilli, thanks to rapid GPU computing. Quantitative live imaging of RPE cell culture with DFFOCT is useful in development of disease models of retinal degeneration.

Paper Details

Date Published: 9 March 2020
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Proc. SPIE 11239, Dynamics and Fluctuations in Biomedical Photonics XVII, 112390O (9 March 2020);
Show Author Affiliations
Kassandra Groux, Institut Langevin Ondes et Images, Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris (France)
Univ. PSL, CNRS (France)
Jules Scholler, Institut Langevin Ondes et Images (France)
Pedro Mecê, Institut Langevin Ondes et Images (France)
Sacha Reichman, Institut de la Vision, Ctr. Hospitalier National d'Opthalmologie des Quinze-Vingts (France)
Valérie Fradot, Institut de la Vision, Ctr. Hospitalier National d'Opthalmologie des Quinze-Vingts (France)
Michel Pâques, Institut de la Vision, Ctr. Hospitalier National d'Opthalmologie des Quinze-Vingts (France)
Claude Boccara, Institut Langevin Ondes et Images (France)
Katharine Grieve, Institut de la Vision, Ctr. Hospitalier National d'Opthalmologie des Quinze-Vingts (France)


Published in SPIE Proceedings Vol. 11239:
Dynamics and Fluctuations in Biomedical Photonics XVII
Valery V. Tuchin; Martin J. Leahy; Ruikang K. Wang, Editor(s)

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