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Proceedings Paper

Intra-tumor heterogeneity of glioblastoma samples revealed by visible light optical coherence microscopy (Conference Presentation)

Paper Abstract

We utilized a visible light optical coherence microscopy imaging (OCM) system to investigate the intra-tumor heterogeneity of glioblastoma samples. Using a broad visible spectrum an axial resolution of 0.8 μm was achieved. Objective lenses with short focal lengths were integrated to achieve high transverse resolution. Large field of view images were acquired using an automatic moving x-y-stage. Attenuation maps were calculated from OCM data and cell-density evaluations were performed from corresponding histological sections. Cell density and attenuation data were evaluated. Our analysis revealed intra-tumor heterogeneity by varying cell densities and attenuation coefficients throughout the tumorous areas.

Paper Details

Date Published: 9 March 2020
Proc. SPIE 11228, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIV, 112281T (9 March 2020); doi: 10.1117/12.2544612
Show Author Affiliations
Antonia Lichtenegger, Medizinische Univ. Wien (Austria)
Johanna Gesperger, Medizinische Univ. Wien (Austria)
Barbara Kiesel, Medizinische Univ. Wien (Austria)
Thomas Roetzer, Medizinische Univ. Wien (Austria)
Pablo Eugui, Medizinische Univ. Wien (Austria)
Danielle J. Harper, Medizinische Univ. Wien (Austria)
Matthias Salas, Medizinische Univ. Wien (Austria)
Marco Augustin, Medizinische Univ. Wien (Austria)
Conrad W. Merkle, Medizinische Univ. Wien (Austria)
Christoph K. Hitzenberger, Medizinische Univ. Wien (Austria)
Georg Widhalm, Medizinische Univ. Wien (Austria)
Adelheid Wöhrer, Medizinische Univ. Wien (Austria)
Bernhard Baumann, Medizinische Univ. Wien (Austria)

Published in SPIE Proceedings Vol. 11228:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIV
Joseph A. Izatt; James G. Fujimoto, Editor(s)

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