Share Email Print

Proceedings Paper

Developing a novel hyperspectral imaging cryomacrotome for whole body fluorescence imaging
Author(s): Boyu Meng; Brook K. Byrd; Dennis J. Wirth; Rendall R. Strawbridge; Scott C. Davis
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

The ability to directly measure whole-body fluorescence can enable tracking of labeled cells, metastatic spread, and drug bio-distribution. We describe the development of a new hyperspectral imaging whole body cryomacrotome designed to acquire 3-D fluorescence volumes in large specimens (whole animals) at high resolution. The use of hyperspectral acquisition provides full spectra at every voxel, enabling spectral decoupling of multiple fluorohpores and autofluorescence. We present examples of tissue spectra and spectral fitting in a rodent glioma xenograft.

Paper Details

Date Published: 21 February 2020
PDF: 5 pages
Proc. SPIE 11219, Visualizing and Quantifying Drug Distribution in Tissue IV, 112190M (21 February 2020); doi: 10.1117/12.2544497
Show Author Affiliations
Boyu Meng, Dartmouth College (United States)
Brook K. Byrd, Dartmouth College (United States)
Dennis J. Wirth, Dartmouth College (United States)
Rendall R. Strawbridge, Dartmouth College (United States)
Scott C. Davis, Dartmouth College (United States)

Published in SPIE Proceedings Vol. 11219:
Visualizing and Quantifying Drug Distribution in Tissue IV
Kin Foong Chan; Conor L. Evans, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?