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Proceedings Paper

An orange-fluorescent dye with long-lasting blinking for single molecule localization microscopy
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Paper Abstract

Single molecule localization microscopy (SMLM) relies on the detection of fluorescence emission from a single fluorophore molecule. Recently, several fluorescent dyes with spontaneous blinking have been reported. Last year, we reported a near-infrared dye with spontaneous blinking and high photostability. Here, we present a new orangefluorescent dye that exhibits spontaneous blinking based on equilibrium between the fluorescent and non-fluorescent forms. We have also ascertained that the new dye can be used to create a super-resolution image of cytoskeletal microtubules. In combination with our near-infrared dye reported last year, the new dye is expected to allow dual-color SMLM.

Paper Details

Date Published: 21 February 2020
PDF: 6 pages
Proc. SPIE 11256, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XII, 112560G (21 February 2020); doi: 10.1117/12.2544492
Show Author Affiliations
Daiki Hara, Sysmex Corp. (Japan)
Yuji Okamoto, Sysmex Corp. (Japan)
Shingo Fujiyama, Sysmex Corp. (Japan)
Youichi Nishikawa, Sysmex Corp. (Japan)
Yoshihiko Norimine, Eisai Co., Ltd. (Japan)
Yuji Kazuta, Eisai Co., Ltd. (Japan)
Takafumi Motoki, Eisai Co., Ltd. (Japan)
Hiroyuki Amino, Eisai Co., Ltd. (Japan)
Shigeki Iwanaga, Sysmex Corp. (Japan)


Published in SPIE Proceedings Vol. 11256:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XII
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

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