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Proceedings Paper

Terbium ion as RNA tag for slide-free histology with deep-ultraviolet excitation fluorescence (Conference Presentation)
Author(s): Yasuaki Kumamoto

Paper Abstract

Terbium ions, Tb3+, fluoresced in a cell at deep-ultraviolet excitation. Intensity of the fluorescence was dropped by RNA decomposition. It was also found that fluorescence intensity of Tb3+ conjugated with DNA increased with strand dissociation. These results indicated specificity of Tb3+ to single-stranded nucleic acid, mostly RNA in cell and tissue. We employed Tb3+ and Hoechst, DNA-specific dyes, for deep-ultraviolet excitation fluorescence imaging. With this imaging, nucleolus, nucleoplasm, and cytoplasm were discriminated. Thanks to the small penetration depth of deep-ultraviolet light into tissue, this imaging modality was useful for slide-free histology and rapid cancer detection in a human lymph node.

Paper Details

Date Published: 9 March 2020
Proc. SPIE 11234, Optical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis, 1123411 (9 March 2020); doi: 10.1117/12.2544487
Show Author Affiliations
Yasuaki Kumamoto, Kyoto Prefectural Univ. of Medicine (Japan)

Published in SPIE Proceedings Vol. 11234:
Optical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis
Robert R. Alfano; Stavros G. Demos; Angela B. Seddon, Editor(s)

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