Share Email Print

Proceedings Paper

Multi-wavelength diffractive beam shaper for rectangular flattop spots in flow cytometer (Conference Presentation)

Paper Abstract

Flow cytometer is a powerful tool for the quantitative analysis of a large population of cells. A key factor determining the measurement accuracy and system stability is the illumination beam. The conventional beam shaper is composed of multiple geometrical optical elements, which should be precisely calibrated to produce elliptical Gaussian beams. It is difficult to control the sizes and positions of the focal spots formed by different wavelengths. A new beam shaping method based on diffractive optical element (DOE) is developed for rectangular flattop spots with multi-wavelength illumination. Its benefits include high simplicity, design flexibility, uniform illumination, and multifunctionality.

Paper Details

Date Published: 9 March 2020
Proc. SPIE 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII, 112430U (9 March 2020); doi: 10.1117/12.2544461
Show Author Affiliations
Jingjing Zhao, Stanford Univ. (United States)
Yong Han, Tsinghua Univ. (China)
Zeheng Jiao, Tsinghua Univ. (China)
Zixi Chao, Tsinghua Univ. (China)
Attila Tárnok, Institut für Medizinische Informatik, Statistik und Epidemiologie (Germany)
Fraunhofer-Institut für Zelltherapie und Immunologie IZI (Germany)
Tsinghua Univ. (China)
Zheng You, Tsinghua Univ. (China)

Published in SPIE Proceedings Vol. 11243:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII
Daniel L. Farkas; Attila Tarnok, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?