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Proceedings Paper

Analysis of coronary microvasculature and myocardial nuclei orientation in embryonic hearts (Conference Presentation)

Paper Abstract

New techniques are needed to study the coronary microvasculature in the embryo and to quantify the differences between healthy and diseased coronary development. We combine our custom optical clearing method (LIMPID), fluorescent staining with DiI and DAPI, and 3D confocal microscopy to visualize the coronary vasculature at two developmental stages (day 9, 13) in quails. We discovered a highly organized coronary vessel network that is aligned with surrounding myocardium cells even at early developmental stages. By characterizing the normal heart vasculature, this experiment provides a baseline for future studies on how diseases affect coronary and myocardial orientation in the embryo.

Paper Details

Date Published: 9 March 2020
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Proc. SPIE 11215, Diagnostic and Therapeutic Applications of Light in Cardiology 2020, 112150T (9 March 2020);
Show Author Affiliations
Maryse Lapierre-Landry, Case Western Reserve Univ. (United States)
Hana Kolesova, Charles Univ. (Czech Republic)
Yehe Liu, Case Western Reserve Univ. (United States)
Michiko Watanabe, Case Western Reserve Univ. (United States)
Michael W. Jenkins, Case Western Reserve Univ. (United States)


Published in SPIE Proceedings Vol. 11215:
Diagnostic and Therapeutic Applications of Light in Cardiology 2020
Kenton W. Gregory M.D.; Laura Marcu, Editor(s)

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