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Proceedings Paper

Nanosecond scanned x-ray sheet imaging for time-resolved luminescence tomography
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Paper Abstract

Biomedical imaging techniques are often limited by the loss of resolution with depth due to light scattering in biological tissue. Beyond a few millimeters in depth, diffuse transport dominates and makes high resolution imaging impossible using conventional techniques. In this work, light sheet imaging using x-ray photons was developed with a keV x-ray source. This partially overcomes this scattering by generating light within tissue at depth. The light excites fluorescent probes that can be used for tumor tracking based upon molecular targeting. Most of the fluorescent probes have a lifetime in the nanosecond range. In this study, the use of a portable linear accelerator delivering 30-ns x-ray pulses was explored. Using x-ray excitable fluorophores, light was generated within a tissue phantom. Image stacks were acquired using an intensified camera (PiMAX4 – Princeton Instrument – USA) placed perpendicularly to the slicing direction of the sample. A solid-state silicon photomultiplier was used to gate acquisition. Although this delayed acquisition slightly, it improved the fluorescence signal-to-noise ratio (SNR). A deconvolution algorithm counteracted the blurring effects of tissue, and image stacks were converted to 3D reconstructions. In summary, nanosecond x-ray pulses can be used to excite fluorophores through radioluminescence phenomenon. Combined with slice imaging, this approach shows promise for time-resolved x-ray luminescence.

Paper Details

Date Published: 25 February 2020
PDF: 6 pages
Proc. SPIE 11224, Optics and Ionizing Radiation, 112240C (25 February 2020); doi: 10.1117/12.2544438
Show Author Affiliations
Arthur Pétusseau, Dartmouth-Hitchcock Medical Ctr. (United States)
Petr Bruza, Dartmouth-Hitchcock Medical Ctr. (United States)
Brian W. Pogue, Dartmouth-Hitchcock Medical Ctr. (United States)

Published in SPIE Proceedings Vol. 11224:
Optics and Ionizing Radiation
Brian W. Pogue, Editor(s)

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