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Proceedings Paper

Closed-loop multiconjugate adaptive optics for microscopy
Author(s): Karen M. Hampson; Jiahe Cui; Matthew Wincott; Richard Lane; Syed Hussain; Kaustubh Banerjee; Pouya Rajaeipour; Hans Zappe; Çağlar Ataman; Martin J. Booth
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Paper Abstract

When imaging a sample, inhomogeneities in refractive index cause blur in the image and decrease resolution. Adaptive optics (AO) is a technique that can correct for the resulting aberrations. The most common implementation of AO uses a single deformable mirror that is conjugate to the pupil. A single pupil-conjugate corrective device provides correction over a limited field of view owing to field-dependent aberrations. To overcome this limitation, an additional specimen-conjugate deformable mirror can be used. However, adding a second reflective correction device significantly increases system complexity. We have developed a closed-loop multiconjugate AO system for field-dependent aberration correction in a confocal fluorescence microscope. A 140-actuator deformable mirror is used in the pupil plane with a custom 37-element transmissive deformable phase plate inserted in a sample-conjugate plane. Both devices are calibrated and controlled in closed-loop using a Shack-Hartmann sensor in combination with an integral control law. The sensor consists of an EMCCD and lenslet array with a 500 μm pitch and a 47 mm focal length. Results from a Drosophila ovary and HeLa cells are presented.

Paper Details

Date Published: 17 February 2020
PDF: 5 pages
Proc. SPIE 11248, Adaptive Optics and Wavefront Control for Biological Systems VI, 1124809 (17 February 2020); doi: 10.1117/12.2544391
Show Author Affiliations
Karen M. Hampson, Univ. of Oxford (United Kingdom)
Jiahe Cui, Univ. of Oxford (United Kingdom)
Matthew Wincott, Univ. of Oxford (United Kingdom)
Richard Lane, Univ. of Oxford (United Kingdom)
Syed Hussain, Univ. of Oxford (United Kingdom)
Kaustubh Banerjee, Univ. of Freiburg (Germany)
Pouya Rajaeipour, Univ. of Freiburg (Germany)
Hans Zappe, Univ. of Freiburg (Germany)
Çağlar Ataman, Univ. of Freiburg (Germany)
Martin J. Booth, Univ. of Oxford (United Kingdom)

Published in SPIE Proceedings Vol. 11248:
Adaptive Optics and Wavefront Control for Biological Systems VI
Thomas G. Bifano; Sylvain Gigan; Na Ji, Editor(s)

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