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Proceedings Paper

Achieving spontaneous super-resolution in a confocal microscope by exploiting super-linear emitters (Conference Presentation)
Author(s): Denitza Denkova; Martin Ploschner; Minakshi Das; Lindsay M. Parker; Xianlin Zheng; Yiqing Lu; Antony Orth; Nicolle H. Packer; James A. Piper

Paper Abstract

We achieve spontaneous 3D super-resolution on a standard confocal microscope by exploiting bio-friendly fluorescent markers with super-linear excitation-emission dependence (upconversion nanoparticles of NaYF4: Yb, Tm). We refer to this approach as upconversion super-linear excitation-emission (uSEE) microscopy. To demonstrate the applicability of the method for biological applications, we image sugar-coated upconversion nanoparticles in neuronal cells and we achieve resolution twice better than the diffraction limit both in lateral and axial directions. We envision that due to the application simplicity of the developed methodological toolbox, uSEE microscopy can be widely incorporated as an everyday super-resolution method in biological laboratories.

Paper Details

Date Published: 9 March 2020
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Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 112460H (9 March 2020); doi: 10.1117/12.2544364
Show Author Affiliations
Denitza Denkova, ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia)
Macquarie Univ. (Australia)
Institute for Bioengineering of Catalonia (Spain)
Martin Ploschner, ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia)
Macquarie Univ. (Australia)
The Univ. of Queensland (Australia)
Minakshi Das, ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia)
Macquarie Univ. (Australia)
Lindsay M. Parker, ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia)
Macquarie Univ. (Australia)
Xianlin Zheng, ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia)
Macquarie Univ. (Australia)
Yiqing Lu, ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia)
Macquarie Univ. (Australia)
Antony Orth, ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia)
RMIT Univ. (Australia)
Nicolle H. Packer, ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia)
Griffith Univ. (Australia)
James A. Piper, ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia)
Macquarie Univ. (Australia)


Published in SPIE Proceedings Vol. 11246:
Single Molecule Spectroscopy and Superresolution Imaging XIII
Ingo Gregor; Felix Koberling; Rainer Erdmann, Editor(s)

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