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Proceedings Paper

3D refractive index tomography with quantitative oblique back-illumination microscopy
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Paper Abstract

We have recently developed quantitative oblique back-illumination microscopy (qOBM), which enables full-field quantitative phase imaging (QPI) of objects embedded in a thick scattering medium. This epi-mode technique makes use of multiple scattering as a source of transmissive illumination from within, allowing for rich structural detail based on optical path delay. We now produce quantitative 3D renderings of index of refraction with sub- cellular detail by computing a 3D transfer function of the entire optical system, including the multiply scattered illumination, and deconvolving it from a vertical stack of phase gradient contrast images. This approach allows truly non-invasive, label-free, tomographic quantitative reconstructions if index of refraction in thick scattering samples including thick tissue samples, at low cost and with simple operation, bringing QPI’s unparalleled access to sub-cellular structural detail to previously unavailable domains of investigation.

Paper Details

Date Published: 17 February 2020
PDF: 8 pages
Proc. SPIE 11245, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII, 1124503 (17 February 2020); doi: 10.1117/12.2544328
Show Author Affiliations
Patrick B. Ledwig, Georgia Institute of Technology & Emory Univ. School of Medicine (United States)
Francisco E. Robles, Georgia Institute of Technology & Emory Univ. School of Medicine (United States)


Published in SPIE Proceedings Vol. 11245:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII
Thomas G. Brown; Tony Wilson; Laura Waller, Editor(s)

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