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Proceedings Paper

Performance requirements for lasers and fibers suited for confocal microscopy and flow cell cytometry applications
Author(s): Volker Melzer; Richard Addy; Huw Major
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Paper Abstract

The availability of high quality laser diode based lasers in the visible range has boosted up many bio-medical applications like Confocal Microscopy and Flow Cytometry. These new lasers are enabling high resolution close to the diffraction limit and improved sensitivity in many biological and medical applications. We explain the most important performance parameters of these lasers which are required to achieve best test results and excellent resolution by using suited laser sources and accessories for these two applications. Some of these parameters are very low noise, low beam pointing, single TEM00 mode like beam quality for diffraction limited spots and the beam shape. Beside the laser sources the use of single mode fibers is essential to achieve excellent laser beam quality and tailored beam shapes in combination with very low beam pointing for highest resolution and superior test results. The paper will explain the important role of Spatial Filtering and how it is working based on apodization in the Fourier space.

Paper Details

Date Published: 17 February 2020
PDF: 12 pages
Proc. SPIE 11231, Design and Quality for Biomedical Technologies XIII, 1123105 (17 February 2020); doi: 10.1117/12.2544290
Show Author Affiliations
Volker Melzer, Qioptiq Photonics GmbH & Co. KG (Germany)
Richard Addy, Qioptiq Photonics Ltd. (United Kingdom)
Huw Major, Qioptiq Photonics Ltd. (United Kingdom)

Published in SPIE Proceedings Vol. 11231:
Design and Quality for Biomedical Technologies XIII
Jeeseong Hwang; Gracie Vargas, Editor(s)

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