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Proceedings Paper

A single particle sensing platform based on 2D gold nanocrystals coated with designable artificial repeat proteins (Conference Presentation)
Author(s): Laureen Moreaud; Janak Prasad; Sirin Celiksoy; Sébastien Voillet; Agathe Urvoas; Marie Valerio-Lepiniec; Philippe Minard; Carsten Soennichsen; Erik Dujardin

Paper Abstract

Shape control of metal nanocrystals with engineered proteins is promising for integrated plasmonics, biosensing, and catalysis applications. We present artificial thermo-stable proteins used as inhibitors during gold nanocrystal growth in order to control the shape and crystallographic facet expression. This synthesis leads to (111)-terminated gold nanocrystals in high yield. The shape and size distribution are tuned towards nanoplates by adjusting synthetic conditions. When biotinylated alpha-Reps are used, the morphosynthetic properties are preserved and streptavidin sensing is observed through a marked 7-nm redshift in dark-field spectra. Single nanoplate streptavidin sensing experiments show high sensitivity (300 nm/RIU).

Paper Details

Date Published: 10 March 2020
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Proc. SPIE 11255, Colloidal Nanoparticles for Biomedical Applications XV, 112550D (10 March 2020); doi: 10.1117/12.2544284
Show Author Affiliations
Laureen Moreaud, Ctr. d'Elaboration de Matériaux et d'Etudes Structurales, CNRS (France)
Janak Prasad, Ctr. d'Elaboration de Matériaux et d'Etudes Structurales, CNRS (France)
Sirin Celiksoy, Johannes Gutenberg Univ. Mainz (Germany)
Sébastien Voillet, Institut de biologie intégrative de la cellule, Univ. Paris-Sud (France)
Agathe Urvoas, Institut de biologie intégrative de la cellule, Univ. Paris-Sud (France)
Marie Valerio-Lepiniec, Institut de biologie intégrative de la cellule, Univ. Paris-Sud (France)
Philippe Minard, Institut de biologie intégrative de la cellule, Univ. Paris-Sud (France)
Carsten Soennichsen, Johannes Gutenberg Univ. Mainz (Germany)
Erik Dujardin, Ctr. d'Elaboration de Matériaux et d'Etudes Structurales, CNRS (France)


Published in SPIE Proceedings Vol. 11255:
Colloidal Nanoparticles for Biomedical Applications XV
Marek Osiński; Antonios G. Kanaras, Editor(s)

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