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Proceedings Paper

Vascular and functional imaging by a fast mechanical scanning dual-wavelength photoacoustic microscopy (PAM)
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Paper Abstract

We present a dual-wavelength fast mechanical scanning optical-resolution photoacoustic microscopy system (OR-PAM) in this paper. Conventional mechanical scanning OR-PAM system takes ~ 30 min for one frame of single wavelength imaging. The acquisition of two wavelengths will double the total imaging time. By using a fast-linear stage, a high signal-to-noise (SNR) optical and acoustic combiner and 2 lasers at different wavelengths, we achieved a B-scan rate of 12 Hz and reduced the acquisition time to ~1.5 min for dual-wavelength imaging. This system can be used in large field optical resolution imaging. Contrast-free vascular and functional imaging can be achieved using this system. Both ex vivo and in vivo imaging results are demonstrated.

Paper Details

Date Published: 17 February 2020
PDF: 6 pages
Proc. SPIE 11240, Photons Plus Ultrasound: Imaging and Sensing 2020, 112403P (17 February 2020); doi: 10.1117/12.2544270
Show Author Affiliations
Renzhe Bi, Singapore Bioimaging Consortium (Singapore)
Malini Olivo, Singapore Bioimaging Consortium (Singapore)
Yang Pu, MicroPhotoAcoustics, Inc. (United States)

Published in SPIE Proceedings Vol. 11240:
Photons Plus Ultrasound: Imaging and Sensing 2020
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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