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Proceedings Paper

FLIm and Raman imaging for detecting micro-environmental changes in bovine pericardium upon genipin cross-linking (Conference Presentation)

Paper Abstract

Genipin cross-linked engineered tissues are 10000 times less toxic than glutaraldehyde cross-linked tissues. Hence, genipin is a better fixative to support the recellularization of tissue-engineered constructs such as vascular grafts. Here, we demonstrate the ability of fiber-based Fluorescence Lifetime Imaging (FLIm) guided Raman spectroscopy to monitor the quality of genipin cross-linked vascular grafts with high speed and specificity. Current results indicate that the fluorescence lifetime of AR-BP shortens upon GE cross-linking. Raman spectroscopy reveals secondary structural changes occurring in the extracellular matrix of pericardia that correspond to Amide I, Amide III and C-C stretch vibrations. We conclude that FLIm guided Raman imaging can detect cross-linking signatures with biochemical specificity and that this imaging modality provides a non-destructive and label-free method to assess the quality of vascular grafts

Paper Details

Date Published: 9 March 2020
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Proc. SPIE 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII, 112431B (9 March 2020); doi: 10.1117/12.2544225
Show Author Affiliations
Tanveer Ahmed Shaik, Leibniz-Institut für Photonische Technologien e.V. (Germany)
Alba Alfonso García, Univ. of California, Davis (United States)
Martin Richter, Friedrich-Schiller-Univ. Jena (Germany)
Florian Korinth, Leibniz-Institut für Photonische Technologien e.V. (Germany)
Anne K. Haudenschild, Univ. of California, Davis (United States)
James F. Mcmasters, Univ. of California, Davis (United States)
Christoph Krafft, Leibniz-Institut für Photonische Technologien e.V. (Germany)
Jürgen Popp, Leibniz-Institut für Photonische Technologien e.V. (Germany)
Laura Marcu, Univ. of California, Davis (United States)


Published in SPIE Proceedings Vol. 11243:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII
Daniel L. Farkas; Attila Tarnok, Editor(s)

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