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Proceedings Paper

High speed 2-photon fluorescence lifetime imaging of protein-protein interactions with a swept array microscope (Conference Presentation)

Paper Abstract

We report the development of a novel massively-parallelised high-speed multifocal FLIM platform with the ability to acquire data 1024 times faster than a conventional TCSPC system. We demonstrate the system performanceFRET imaging of the fluorescent protein biosensor PercivalHR in iPSC derived neurons to measure the dynamic concentration of ADP/ATP in live cells. The advantages and performance envelope of the system will be shown and the potential for further applications explored.

Paper Details

Date Published: 9 March 2020
Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112441A (9 March 2020); doi: 10.1117/12.2544220
Show Author Affiliations
Simon M. Ameer-Beg, King's College London (United Kingdom)
Simon P. Poland, King's College London (United Kingdom)
Thomas Kavanagh, King's College London (United Kingdom)
James A. Levitt, King's College London (United Kingdom)
Robert K. Henderson, The Univ. of Edinburgh (United Kingdom)
Hanning Mai, The Univ. of Edinburgh (United Kingdom)
Andrea Serio, King's College London (United Kingdom)
Conor Treacy, King's College London (United Kingdom)
Kelly O'Toole, King's College London (United Kingdom)
Richard Walker, Photon Force Ltd. (United Kingdom)

Published in SPIE Proceedings Vol. 11244:
Multiphoton Microscopy in the Biomedical Sciences XX
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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