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Proceedings Paper

Imaging the airy way: Advanced beam shaping for light-sheet microscopy at depth (Conference Presentation)

Paper Abstract

Attenuation of optical fields owing to scattering and absorption limits the penetration depth into tissue. Whilst aberration correction may be used this is difficult to implement over a large field of view in heterogeneous tissue. Recently, the novel approach of attenuation-compensation of propagation-invariant light fields has shown increase in depth penetration for light-sheet microscopy. Here we show this powerful approach may be implemented in a facile manner utilizing a graded neutral density filter circumventing the need for expensive beam shaping apparatus. A ‘gold standard’ system utilizing a spatial light modulator for beam shaping is used to benchmark our low-cost implementation.

Paper Details

Date Published: 9 March 2020
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Proc. SPIE 11245, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII, 112450J (9 March 2020); doi: 10.1117/12.2544205
Show Author Affiliations
Madhu Veettikazhy, Technical Univ. of Denmark (Denmark)
Jonathan Nylk, Univ. of St. Andrews (United Kingdom)
Federico M. Gasparoli, Univ. of St. Andrews (United Kingdom)
Adrià Escobet-Montalbán, Univ. of St. Andrews (United Kingdom)
Anders K. Hansen, Technical Univ. of Denmark (Denmark)
Dominik Marti, Technical Univ. of Denmark (Denmark)
Peter E. Andersen, Technical Univ. of Denmark (Denmark)
Kishan Dholakia, Univ. of St. Andrews (United Kingdom)


Published in SPIE Proceedings Vol. 11245:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII
Thomas G. Brown; Tony Wilson; Laura Waller, Editor(s)

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