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Proceedings Paper

Fast volumetric mapping of human brain slices
Author(s): Luca Pesce; Annunziatina Laurino; Vladislav Gavryusev; Giacomo Mazzamuto; Giuseppe Sancataldo; Marina Scardigli; Matteo Roffilli; Ludovico Silvestri; Irene Costantini; Francesco Saverio Pavone
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Paper Abstract

We still lack a detailed map of the anatomical disposition of neurons in the human brain. A complete map would be an important step for deeply understanding the brain function, providing anatomical information useful to decipher the neuronal pattern in healthy and diseased conditions. Here, we present several important advances towards this goal, obtained by combining a new clearing method, advanced Light Sheet Microscopy and automated machine-learning based image analysis. We perform volumetric imaging of large sequentially stained human brain slices, labelled for two different neuronal markers NeuN and GAD67, discriminating the inhibitory population and reconstructing the brain connectivity.

Paper Details

Date Published: 21 February 2020
PDF: 4 pages
Proc. SPIE 11226, Neural Imaging and Sensing 2020, 112260B (21 February 2020); doi: 10.1117/12.2544133
Show Author Affiliations
Luca Pesce, Univ. degli Studi di Firenze (Italy)
LENS - Lab. Europeo di Spettroscopie Non-Lineari (Italy)
Annunziatina Laurino, Univ. degli Studi di Firenze (Italy)
LENS - Lab. Europeo di Spettroscopie Non-Lineari (Italy)
Vladislav Gavryusev, Univ. degli Studi di Firenze (Italy)
LENS - Lab. Europeo di Spettroscopie Non-Lineari (Italy)
Giacomo Mazzamuto, Univ. degli Studi di Firenze (Italy)
Istituto Nazionale di Ottica, Consiglio Nazionale delle Ricerche (Italy)
Giuseppe Sancataldo, Univ. degli Studi di Firenze (Italy)
LENS - Lab. Europeo di Spettroscopie Non-Lineari (Italy)
Marina Scardigli, Univ. degli Studi di Firenze (Italy)
LENS - Lab. Europeo di Spettroscopie Non-Lineari (Italy)
Matteo Roffilli, Bioretics srl (Italy)
Ludovico Silvestri, Univ. degli Studi di Firenze (Italy)
LENS - Lab. Europeo di Spettroscopie Non-Lineari (Italy)
Istituto Nazionale di Ottica, Consiglio Nazionale delle Ricerche (Italy)
Irene Costantini, Univ. degli Studi di Firenze (Italy)
LENS - Lab. Europeo di Spettroscopie Non-Lineari (Italy)
Francesco Saverio Pavone, Univ. degli Studi di Firenze (Italy)
LENS - Lab. Europeo di Spettroscopie Non-Lineari (Italy)
Istituto Nazionale di Ottica, Consiglio Nazionale delle Ricerche (Italy)


Published in SPIE Proceedings Vol. 11226:
Neural Imaging and Sensing 2020
Qingming Luo; Jun Ding; Ling Fu, Editor(s)

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