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Proceedings Paper

Shining a light on antibiotic selection: optimised live/dead fluorescence spectrometry for rapid antimicrobial susceptibility testing (Conference Presentation)
Author(s): Julia Robertson; Fang Ou; Cushla M. McGoverin; Frédérique Vanholsbeeck; Simon Swift

Paper Abstract

Antibiotic resistance is a serious threat to public health. The empiric use of the wrong antibiotic occurs due to urgency in treatment combined with slow, culture-based diagnostic techniques. Inappropriate antibiotic choice can promote the development of antibiotic resistance. We investigated live/dead spectrometry using a fluorimeter (Optrode) as a rapid alternative to culture-based techniques through application of the LIVE/DEAD® BacLightTM Bacterial Viability Kit. Killing was detected by the Optrode in near real-time when E. coli was treated with ampicillin and stained with SYTO 9 and/or PI but only when a suitable concentration, bacterial growth phase, and treatment time was used.

Paper Details

Date Published: 10 March 2020
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Proc. SPIE 11223, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020, 112230E (10 March 2020); doi: 10.1117/12.2544082
Show Author Affiliations
Julia Robertson, The Univ. of Auckland (New Zealand)
Fang Ou, The Univ. of Auckland (New Zealand)
Cushla M. McGoverin, The Univ. of Auckland (New Zealand)
Frédérique Vanholsbeeck, The Univ. of Auckland (New Zealand)
Simon Swift, The Univ. of Auckland (New Zealand)


Published in SPIE Proceedings Vol. 11223:
Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020
Tianhong Dai; Jürgen Popp; Mei X. Wu M.D., Editor(s)

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