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Proceedings Paper

Stimulated Raman scattering - vibrational imaging in cells, tissues and model organisms (Conference Presentation)
Author(s): Volker Schweikhard

Paper Abstract

Stimulated Raman Scattering (SRS) microscopy is a powerful nonlinear optical microscopy technique that images biological structures by exploiting the characteristic, vibrational contrast of the sample molecules. SRS provides a rich, chemically specific and biophysical image contrast that is in many ways complementary to the molecular contrast of fluorescence microscopy. Here, we present a range of applications of SRS, including label-free morphochemical imaging in model organisms, the characterization of organoids and spheroids, and investigations of brain tissues for neurodegenerative disease research. We show specifically that SRS can provide novel insights into the biophysical properties and biochemical composition of Amyloid-β plaques in a mouse model of Alzheimer’s disease. Our results highlight the potential of SRS to contribute to a deeper understanding of cell and tissue biology, and to serve as a powerful tool for preclinical and translational research.

Paper Details

Date Published: 10 March 2020
Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 112521J (10 March 2020); doi: 10.1117/12.2544013
Show Author Affiliations
Volker Schweikhard, Leica Microsystems CMS GmbH (Germany)

Published in SPIE Proceedings Vol. 11252:
Advanced Chemical Microscopy for Life Science and Translational Medicine
Ji-Xin Cheng; Wei Min; Garth J. Simpson, Editor(s)

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