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Proceedings Paper

Femtosecond transient absorption spectroscopy to study the effects of low irradiance light on cytochrome c and cytochrome c reductase
Author(s): Sean P. O'Connor; Samantha M. Powell; John M. Rickman; Gary D. Noojin; Nathaniel J. Pope; Marlan O. Scully; Michael L. Denton; Vladislav V. Yakovlev
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Paper Abstract

Photobiomodulation (PBM) describes the enhancement of cellular functions following exposure to low irradiance visible or NIR light. Although these effects are not well understood, PBM has been shown to enhance the synthesis of ATP so the mitochondrion is the hypothesized target for the processes of photobiomodulation. More specifically, cytochromecontaining enzyme complexes of the electron transport chain (ETC) in the mitochondria are expected to be the primary photoabsorbers of the light thought to induce PBM. Recently, our group found light-induced changes in the activity of complex III (cytochrome c reductase) in isolated mitochondria. In this study, we use femtosecond transient absorption spectroscopy (TAS) to study the excited state dynamics of the electronic transitions in complex III as well as reduced cytochrome c. To investigate the potential for inducing PBM effects in these proteins, TAS experiments are performed without, and with, low irradiance light exposures during the scanning procedure choosing from blue (450 nm), red (635 nm), and near-infrared (808 nm) laser diodes. The TAS experiments with and without light exposures during the procedure are compared to determine if PBM effects were induced. Understanding illumination induced changes in the excited state dynamics of proteins can help to better characterize the molecular processes caused by PBM and lead to a more optimized treatment for the enhancement of human performance and therapy.

Paper Details

Date Published: 11 March 2020
PDF: 11 pages
Proc. SPIE 11221, Mechanisms of Photobiomodulation Therapy XV, 112210D (11 March 2020); doi: 10.1117/12.2544012
Show Author Affiliations
Sean P. O'Connor, Texas A&M Univ. (United States)
Samantha M. Powell, Air Force Research Lab. (United States)
John M. Rickman, SAIC (United States)
Gary D. Noojin, SAIC (United States)
Nathaniel J. Pope, Oak Ridge Institute for Science and Education (United States)
Marlan O. Scully, Texas A&M Univ. (United States)
Baylor Univ. (United States)
Michael L. Denton, Air Force Research Lab. (United States)
Vladislav V. Yakovlev, Texas A&M Univ. (United States)

Published in SPIE Proceedings Vol. 11221:
Mechanisms of Photobiomodulation Therapy XV
Michael R. Hamblin; James D. Carroll; Praveen Arany, Editor(s)

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