Share Email Print
cover

Proceedings Paper

Digital histology of tissue with Mueller polarimetric microscopy (Conference Presentation)

Paper Abstract

Tissue-based diagnostic relies on histological analysis of tissue cuts by a pathologist who links data derived from microscopy image content to a specific disease. We explored the potential of Mueller microscopy combined with density-based spatial clustering algorithm for applications with noise for the automated analysis of tissue histological cuts. Mueller microscopic images of unstained artificial human skin histological cuts were analyzed by applying differential Mueller matrix decomposition. Scalar retardance, depolarization and total transmitted intensity images were used for the automated segmentation of microscopic images. An algorithm of polarimetric data post-processing which mitigates the impact of tissue thickness fluctuations was suggested.

Paper Details

Date Published: 10 March 2020
PDF
Proc. SPIE 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020, 1125111 (10 March 2020); doi: 10.1117/12.2543976
Show Author Affiliations
Hee Ryung Lee, Lab. de Physique des Interfaces et des Couches Minces, CNRS (France)
Pengcheng Li, Tsinghua Univ. (China)
Hui Ma, Tsinghua Univ. (China)
Christian Lotz, Universitätsklinikum Würzburg (Germany)
Fraunhofer-Institut für Silicatforschung ISC (Germany)
Florian Kai Groeber-Becker, Universitätsklinikum Würzburg (Germany)
Fraunhofer-Institut für Silicatforschung ISC (Germany)
Sofia Dembski, Universitätsklinikum Würzburg (Germany)
Fraunhofer-Institut für Silicatforschung ISC (Germany)
Razvigor Ossikovski, Lab. de Physique des Interfaces et des Couches Minces, CNRS (France)
Tatiana Novikova, Lab. de Physique des Interfaces et des Couches Minces, CNRS (France)


Published in SPIE Proceedings Vol. 11251:
Label-free Biomedical Imaging and Sensing (LBIS) 2020
Natan T. Shaked; Oliver Hayden, Editor(s)

© SPIE. Terms of Use
Back to Top
PREMIUM CONTENT
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?
close_icon_gray