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Proceedings Paper

Fingerprint–to-CH stretch region high spectral resolution Stimulated Raman Scattering microscopy with an Acousto Optical Tunable Filter (Conference Presentation)
Author(s): Sergey P. Laptenok; Luca Genchi; Vijayakumar P. Rajamanickam; Andrea Bertoncini; Alessandro Giammona; Carlo Liberale

Paper Abstract

We present a novel design for a Stimulated Raman Scattering (SRS) microscope based on a dual beam femtosecond laser in combination with spectral shaping through a fast and narrowband Acousto Optical Tunable Filter. This configuration allows the measurement of broad SRS spectra, all the way from fingerprint region to CH stretch region without any modification of the optical setup. High spectral resolution over a broad spectral region allows label-free quantitative imaging of biological samples. We will show the application of our SRS system to a quantitative study of lipid droplets in colon Cancer Stem Cells.

Paper Details

Date Published: 10 March 2020
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Proc. SPIE 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020, 112511B (10 March 2020); doi: 10.1117/12.2543972
Show Author Affiliations
Sergey P. Laptenok, King Abdullah Univ. of Science and Technology (Saudi Arabia)
Luca Genchi, King Abdullah Univ. of Science and Technology (Saudi Arabia)
Vijayakumar P. Rajamanickam, King Abdullah Univ. of Science and Technology (Saudi Arabia)
Andrea Bertoncini, King Abdullah Univ. of Science and Technology (Saudi Arabia)
Alessandro Giammona, King Abdullah Univ. of Science and Technology (Saudi Arabia)
Carlo Liberale, King Abdullah Univ. of Science and Technology (Saudi Arabia)


Published in SPIE Proceedings Vol. 11251:
Label-free Biomedical Imaging and Sensing (LBIS) 2020
Natan T. Shaked; Oliver Hayden, Editor(s)

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