With the rise of antibiotic resistance, phage therapy is seen as a promising alternative to cure infection to multiresistant bacteria strains. However, phage susceptibility tests currently carried out are time-consuming and are not compliant with the automated environment of hospital laboratories. In this work, we present a method for phage susceptibility testing through optical density measurement with the use of lensless imaging technique. Using a 3.3 cm2 area CANON sensor and a custom test card, we are able to simultaneously monitor the bacterial growth or inhibition of multiple bacterial/phage samples and to provide reliable results in less than 4 hour.

Date Published: 10 March 2020
PDF
Proc. SPIE 11223, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020, 112230D (10 March 2020); doi: 10.1117/12.2543785

Published in SPIE Proceedings Vol. 11223:
Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020
Tianhong Dai; Jürgen Popp; Mei X. Wu M.D., Editor(s)