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Proceedings Paper

Easy two-photon image-scanning microscopy with SPAD array and blind image reconstruction (Conference Presentation)

Paper Abstract

We propose a straightforward implementation of two-photon image scanning microscopy (2PE-ISM) that, by leveraging our recently introduced single-photon avalanche diode (SPAD) array detector and a novel blind image reconstruction algorithm is shown to dramatically improve the optical resolution of two-photon imaging, in various test samples. We show how our computational ISM approach is able to adapt to changing imaging conditions, thus ensuring optimal image quality. We also show how our recently introduced blind deconvolution approaches can be integrated into the image reconstruction workflow to further improve the image quality.

Paper Details

Date Published: 9 March 2020
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Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112440X (9 March 2020); doi: 10.1117/12.2543774
Show Author Affiliations
Sami V. Koho, Istituto Italiano di Tecnologia (Italy)
Eli Slenders, Istituto Italiano di Tecnologia (Italy)
Giorgio Tortarolo, Istituto Italiano di Tecnologia (Italy)
Marco Castello, Istituto Italiano di Tecnologia (Italy)
Mauro Buttafava, Politecnico di Milano (Italy)
Federica A. Villa, Politecnico di Milano (Italy)
Elena Tcarenkova, Univ. of Turku (Finland)
Marcel Ameloot, Univ. Hasselt (Belgium)
Paolo Bianchini, Istituto Italiano di Tecnologia (Italy)
Colin J. R. Sheppard, Istituto Italiano di Tecnologia (Italy)
Alberto Diaspro, Istituto Italiano di Tecnologia (Italy)
Alberto Tosi, Politecnico di Milano (Italy)
Giuseppe Vicidomini, Istituto Italiano di Tecnologia (Italy)


Published in SPIE Proceedings Vol. 11244:
Multiphoton Microscopy in the Biomedical Sciences XX
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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