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Proceedings Paper

A simple, compact and robust phase and fluorescence microscope for cell cycle study (Conference Presentation)
Author(s): Ondrej Mandula; Cedric Allier; Dainel Fiole; Jean-Philippe Kleman; Francoise Lacroix; Lionel Hervé; Sophie Morales

Paper Abstract

We designed a simple, compact and robust microscope for phase and fluorescent imaging. No mechanical movement of neither sample nor objective or any other part of the system is needed to change between the phase-contrast and fluorescence modality. We can observe a thousand cells in parallel in a single field of view with resolution down to 2 µm. We demonstrate the system on a FUCCI marked HeLa cell culture observed over several days directly in the standard incubator. We compare the obtained statistics to the flow cytometer data and show that we can produce a statistically relevant time-resolved measurement

Paper Details

Date Published: 9 March 2020
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Proc. SPIE 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII, 112430P (9 March 2020); doi: 10.1117/12.2543764
Show Author Affiliations
Ondrej Mandula, Univ. Grenoble Alpes (France)
Lab. d'Electronique de Technologie de l'Information (France)
Cedric Allier, Univ. Grenoble Alpes (France)
Lab. d'Electronique de Technologie de l'Information (France)
Dainel Fiole, Univ. Grenoble Alpes (France)
Lab. d'Electronique de Technologie de l'Information (France)
DTBS-LSIV (France)
Jean-Philippe Kleman, Univ. Grenoble Alpes (France)
Institut de Biologie Structurale, CNRS (France)
Francoise Lacroix, Univ. Grenoble Alpes (France)
Institut de Biologie Structurale, CNRS (France)
Lionel Hervé, Univ. Grenoble Alpes (France)
Lab. d'Electronique de Technologie de l'Information (France)
DTBS-LSIV (France)
Sophie Morales, Univ. Grenoble Alpes (France)
Lab. d'Electronique de Technologie de l'Information (France)
DTBS-LSIV (France)


Published in SPIE Proceedings Vol. 11243:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII
Daniel L. Farkas; Attila Tarnok, Editor(s)

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