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Proceedings Paper

All-optical correlative micro-spectroscopies in the investigation of stromal collagen morpho-mechanics
Author(s): Raffaella Mercatelli; Sara Mattana; Laura Capozzoli; Fulvio Ratto; Francesca Rossi; Roberto Pini; Daniele Fioretto; Francesco S. Pavone; Silvia Caponi; Riccardo Cicchi
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Paper Abstract

Tissue morpho-mechanics is gaining increasing in various fields, because it targets the relationship between morphological features and mechanical properties in biological tissues, which plays an important role in various fields including biology, medicine, pathology, tissue engineering, and regenerative medicine. The intimate connection between morphological, biochemical and mechanical properties in biological tissues requires a multimodal correlative approach for their exhaustive investigation. In this study, we used Second-Harmonic Generation in combination with Brillouin and Raman micro-spectroscopy in order to correlate collagen morphology at the ultrastructural level with its biomechanical and biochemical features. In particular, by imaging human corneal tissue samples with our multimodal approach, we demonstrated that the peculiar mechanical properties of corneal lamellae in the anterior portion of the corneal stroma are due to a different supramolecular organization, rather than to a different biochemical composition. This result opens new insights in the study and interpretation of corneal biomechanics thanks to the possibility of non-invasively correlating lamellar morphology with visco-elastic properties. The proposed method opens the way to the non-invasive assessment of corneal morpho-mechanics and holds the potential to be used for both diagnostic and follow-up purposes in pathologies that affect corneal biomechanics.

Paper Details

Date Published: 19 February 2020
PDF: 4 pages
Proc. SPIE 11218, Ophthalmic Technologies XXX, 112180U (19 February 2020); doi: 10.1117/12.2543742
Show Author Affiliations
Raffaella Mercatelli, National Institute of Optics, CNR (Italy)
Aerospazio Tecnologie s.r.l. (Italy)
Sara Mattana, National Institute of Optics, CNR (Italy)
Univ. of Florence (Italy)
Laura Capozzoli, Institute of Chemistry of Organometallic Compounds, CNR (Italy)
Fulvio Ratto, Institute of Applied Physics "Nello Carrara", CNR (Italy)
Francesca Rossi, Institute of Applied Physics "Nello Carrara", CNR (Italy)
Roberto Pini, Institute of Applied Physics "Nello Carrara", CNR (Italy)
Daniele Fioretto, Univ. of Perugia (Italy)
Ctr. of Excellence for Innovative Nanostructured Material (Italy)
Francesco S. Pavone, National Institute of Optics, CNR (Italy)
Univ. of Florence (Italy)
European Lab. for Non-linear Spectroscopy (Italy)
Silvia Caponi, Ctr. of Excellence for Innovative Nanostructured Material (Italy)
Institute of Materials, CNR (Italy)
Univ. of Perugia (Italy)
Riccardo Cicchi, National Institute of Optics, CNR (Italy)
European Lab. of Non-linear Spectroscopy (Italy)


Published in SPIE Proceedings Vol. 11218:
Ophthalmic Technologies XXX
Fabrice Manns; Arthur Ho; Per G. Söderberg, Editor(s)

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