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Nanoconcentrations of 1,9-dimethylmethylene blue (DMMB) associated to laser, LED or polarized light are highly effective on AmPDT carried out in aerobes and aerotolerant anaerobes Gram-positive bacteria
Author(s): Antonio L. B. Pinheiro; Fernando J. P. Sampaio; Darcy de A. Santos; Pedro Jorge L. Crugeira; Susana C. P. S. de Oliveira; Juliana S. C. Monteiro; Sandra R. C. A. Fagnani; Iago P. F. Nunes; Luiz G. P. Soares; Paulo Fernando de Almeida
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Paper Abstract

Antimicrobial Photodynamic Therapy (AmPDT) is an alternative to conventional treatments of local infections such as the use of antibiotics, which may lead to the development of resistance. AmPDT in addition of requiring the use of a photosensitizer it also needs a light source executed. In search of an efficient and cheap procedure, we aimed to assess the efficacy of using 1,9-dimethylmethylene blue (DMMB) as photosensitizer associated with the use of either Laser (λ660 nm), LED (λ632 ± 2 nm) or a multispectral polarized light (λ400 – 2000 nm) using different energy densities to kill E. faecalis (Laser or LED) or S. aureus (PL) in vitro. For this, 300 (S. aureus) or 3.32 ηg/ml (E. faecalis) of DMBB and energy densities of 5 (PL) or 18 J/cm2 (Laser/LED) were used. Five minutes of pre-incubation time was used. CFU count (S. aureus) or the most probable number method (MPN) (E. faecalis) were used as assessment methods. The results showed a 99.97 % reduction on CFU counts of S. aureus and 99.999998 % reduction on E. faecalis (using both light sources). It is concluded that AmPDT carried out with 1,9-dimethylmethylene blue associated with either Laser, LED or Polarized light was efficacious on the reduction, in vitro, of the counts of aerobes and aerotolerant anaerobes Gram-positive bacteria.

Paper Details

Date Published: 11 March 2020
PDF: 6 pages
Proc. SPIE 11221, Mechanisms of Photobiomodulation Therapy XV, 112210P (11 March 2020); doi: 10.1117/12.2543732
Show Author Affiliations
Antonio L. B. Pinheiro, Univ. Federal da Bahia (Brazil)
National Institute of Basic Optics and Applied to Life Sciences (Brazil)
Brazil Univ. Scientific and Technological Institute (Brazil)
Fernando J. P. Sampaio, Univ. Federal da Bahia (Brazil)
National Institute of Basic Optics and Applied to Life Sciences (Brazil)
Darcy de A. Santos, Univ. Federal da Bahia (Brazil)
Federal Univ. of the Reconcavo Bahiano (Brazil)
Pedro Jorge L. Crugeira, Univ. Federal da Bahia (Brazil)
Susana C. P. S. de Oliveira, Univ. Federal da Bahia (Brazil)
Juliana S. C. Monteiro, Univ. Federal da Bahia (Brazil)
Sandra R. C. A. Fagnani, Univ. Federal da Bahia (Brazil)
Iago P. F. Nunes, Univ. Federal da Bahia (Brazil)
Luiz G. P. Soares, Univ. Federal da Bahia (Brazil)
Paulo Fernando de Almeida, Univ. Federal da Bahia (Brazil)


Published in SPIE Proceedings Vol. 11221:
Mechanisms of Photobiomodulation Therapy XV
Michael R. Hamblin; James D. Carroll; Praveen Arany, Editor(s)

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